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The Experimental Method Of Chondroitin Sulfate

Qufu Focuschem Trading Co.,Ltd | Updated: Oct 25, 2017

The purified antibody package is made of the micro-pore plate, the solid-phase carrier is added to the micro-pore of the anti-HPA antibody by adding the specimen or standard product, the Biotin anti-HPA antibody, the HRP labeled affinity element, after thoroughly washing, and then using the substrate TMB color.Chondroitin Sulfate 

The TMB is converted into blue under the catalysis of peroxidase and converted into the final yellow under the action of acid. The depth of the color is positively correlated with the HPA in the sample. The absorbance (OD value) was measured at 450nm wavelength by the enzyme-labeled instrument, and the sample concentration was calculated.Chondroitin Sulfate

Serum: All blood specimens should be placed at room temperature for 2 hours or 4 ℃ after the overnight 1000xg centrifugal 20 minutes, take supernatant can be detected, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw. Plasma: EDTA or heparin can be used as anticoagulant, the specimen collected within 30 minutes after 2-8°c1000xg centrifugation for 15 minutes, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw. Cell culture supernatant or other biological specimens: please 1000XG centrifugation 20 minutes, take supernatant can be detected, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw.Chondroitin Sulfate

Each hole is added to terminate the solution 50μl, the reaction is terminated, and the blue is turned yellow. The order of the termination fluid shall be the same as the addition order of the substrate liquid. In order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time. The optical density (OD) of each hole is measured in the 450nm wavelength by the enzyme-linked instrument. The test is carried out immediately after the liquid is added. Reagent preparation: All reagents must reach room temperature before use, please immediately follow the instructions to save the reagent. In the experiment, please use disposable suction head to avoid cross contamination.Chondroitin Sulfate