Academic Center

Home > Academic Center > Content

The Repeated Sample Process Of Chondroitin Sulfate

Qufu Focuschem Trading Co.,Ltd | Updated: Nov 03, 2017

The purified antibody package is made of micro-porous plate, the solid-phase carrier is prepared into the micro-hole of the anti CS antibody, the sample or standard product, the Biotin anti-CS antibody, the HRP labeled affinity element are added in order, after thorough washing, the substrate TMB color.Chondroitin Sulfate 

The TMB is converted into blue under the catalysis of peroxidase and converted into the ultimate yellow under the action of acid. The depth of the color is positively correlated with the CS in the sample. The absorbance (OD value) was measured at 450nm wavelength by the enzyme-labeled instrument, and the sample concentration was calculated.Chondroitin Sulfate

Serum: All blood specimens should be placed at room temperature for 2 hours or 4 ℃ after overnight $number x G centrifugation 20 minutes, take supernatant can be detected, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw. Plasma: EDTA or heparin can be used as anticoagulant, the specimen collected within 30 minutes after 2-8°c $number x G centrifugation for 15 minutes, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw. Cell culture supernatant or other biological specimens: please $number x G 20 minutes, take the supernatant can be detected, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw.Chondroitin Sulfate

When adding or adding reagents, please note that when drawing specimen/standard, enzyme binding or substrate, the time interval between the first hole and the last hole plus sample is too large, it will lead to different "incubation" time, which obviously affects the correctness and repeatability of the measurement value. A sample addition time (including standard and all samples) is best controlled within 10 minutes, such as the number of specimens, the recommended use of multi-channel liquid.Chondroitin Sulfate

The substrate please avoid light preservation, in storage and Wen to prevent direct light irradiation. It is suggested that two-hole measurement should be established to ensure the correctness of the test results. If the material content in the specimen is too high, please dilute it before you measure it, then multiply it at last. Cell culture supernatant or other biological specimens: please 1000XG centrifugation 20 minutes, take supernatant can be detected, or the specimen placed in -20℃ or-80 ℃ preservation, but should avoid repeated freeze-thaw.Chondroitin Sulfate